The ability of aberrant methylation events to serve as diagnostic and prognostic markers is being appreciated for many cancers, including
prostate cancer. Using quantitative MethyLight technology, we evaluated the relationship between HOXD3 methylation and clinicopathological parameters including biochemical recurrence, pathological stage, Gleason score (GS), and Gleason check details pattern in a series of 232 radical prostatectomies performed between 1998 and 2001. HOXD3 methylation was significantly greater in GS 7 cancers vs GS <= 6 cancers (P-value <0.001) as well as pT3/pT4 vs pT2 cancers (P-value <0.001). The proportion of cases with high methylation in GS 7 vs <= GS 6 and pT3/pT4 vs pT2 were also significantly different (P-values -0.002 and 0.005, respectively). There were also significant increases in methylation from Gleason pattern 2-3 and from pattern 3 to 4/5 (paired t-test P-values = 0.01 and <0.001, respectively), whereas methylation from CX-5461 ic50 lymph node metastases was decreased when compared with matched tumor tissue (P-value = 0.029). HOXD3 methylation
was associated with biochemical recurrence in univariate analysis (P-value = 0.043) and showed evidence for interaction with pathological stage as a predictor variable in Cox regression analysis (P-value -0.028). The results indicate that HOXD3 methylation distinguishes low-grade prostate cancers from intermediate and high-grade ones and may also have prognostic diglyceride value when considered together with pathological stage. Laboratory Investigation (2010) 90, 1060-1067; doi: 10.1038/labinvest.2010.57; published online 8 March 2010″
“We
present an extensive investigation (24 experiments) of a new case of prosopagnosia following right unilateral damage, GG, with the aim of addressing two classical issues: (1) Can a visual recognition impairment truly be specific to faces? (2) What is the nature of acquired prosopagnosia? We show that GG recognizes nonface objects perfectly and quickly, even when it requires fine-grained analysis to individualize these objects. He is also capable of perceiving objects and faces as integrated wholes, as indicated by normal Navon effect, 3D-figures perception and perception of Mooney and Arcimboldo face stimuli. However, the patient could not perceive individual faces holistically, showing no inversion, composite, or whole-part advantage effects for faces. We conclude that an occipito-temporal tight hemisphere lesion may lead to a specific impairment of holistic perception of individual items, a function that appears critical for normal face recognition but not for object recognition. (C) 2010 Elsevier Ltd. All rights reserved.”
“Spectral cytopathology (SCP) is a novel spectroscopic method for objective and unsupervised classification of individual exfoliated cells. The limitations of conventional cytopathology are well recognized within the pathology community.