Copyright (C) 2007 John Wiley & Sons, Ltd.”
“Diaphanous-related formin, mDia, is an actin nucleation/polymerization factor functioning downstream of the small GTPase Rho. Although Rho is critically involved in cytokinesis, it remains elusive how Rho effectors and other regulators of cytoskeletons work together to accomplish this process. Here we focused on mDia2, an mDia isoform involved in cytokinesis of NIH 3T3 cells, and analyzed mechanisms of its localization in cytokinesis. We found that JNJ-26481585 molecular weight targeting

of mDia2 to the cleavage furrow requires not only its binding to RhoA but also its diaphanous-inhibitory domain ( DID). We then performed pulldown assays using a fragment containing the latter domain as a bait and identified anillin as a novel mDia2 interaction partner. The anillin-binding is competitive with the diaphanous autoregulatory domain ( DAD) of mDia2 in its autoinhibitory interaction. A series of RNA interference and functional rescue experiments has revealed that, in addition to the Rho GTPase-mediated activation, the interaction between mDia2 and anillin is required for the localization and function of mDia2 in cytokinesis.”
“Physically crosslinked chitosan-g-poly(vinyl alcohol) hydrogels with controllable graft percent

were prepared in three steps. Poly(vinyl acetate) (PVAc) was first grafted onto chitosan via radical copolymerization. Then the copolymer was converted into chitosan-g-poly(vinyl alcohol) check details by alcoholysis reaction. The graft percent of poly (vinyl alcohol) (PVA) could be tailored by the reaction conditions such as the solvent composition, the concentration of initiator and the amount of monomer added. Finally, Chitosan-g-PVA hydrogels were formed by freezing-thawing cycles. The structure of graft copolymers was verified with FTIR. Both XRD analysis and contact angle test showed that the difference of crystallinity and hydrophilicity among chitosan, chitosan-g-PVAc and chitosan-g-PVA was evident. It was found that the maximum swelling ratios of chitosan-g-PVA hydrogels containing 37.6% and 46.2% PVA were 3.48

+/- 0.11 and 4.40. +/- 0.14 at pH 1.2, 1.90 +/- 0.13 and 2.80 +/- 0.15 at pH 7.4, GSK2245840 in vitro respectively. This suggested the hydrogel was pH-sensitive, and its swelling behavior could be tailored with the graft percent of PVA. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 117: 2946-2950, 2010″
“Objectives.-The purpose of this study was to directly compare the pharmacokinetic (PK) profile of 22-mg sumatriptan powder delivered intranasally with a novel Breath Powered device (11mg in each nostril) vs a 20-mg sumatriptan liquid nasal spray, a 100-mg oral tablet, and a 6-mg subcutaneous injection.\n\nBackground.-A prior PK study found that low doses of sumatriptan powder delivered intranasally with a Breath Powered device were efficiently and rapidly absorbed.