In the present study, we demonstrate that a major component of i-Extract and withanone (i-Factor) protected the normal human fibroblasts against the toxicity caused
by withaferin A. It increased the in vitro division potential of normal human cells that appeared to be mediated by decreased accumulation of molecular damage, downregulation of the senescence-specific beta-galactosidase activity and the senescence marker protein, p21(WAF-1), protection against oxidative damage, and induction of proteasomal activity. To the best of our knowledge, we provide the first example of phytochemical(s) (i-Extract and withanone) that have both anticancer and antiaging activities and point Tubastatin A in vitro to the molecular link between aging and cancer.”
“To date, Alzheimer disease (AD) is still difficult to be diagnosed in its earliest stage. The cell cycle aberrations may be the earliest neuropathological events detected in AD thus far. The cell cycle regulatory failure in AD occurring at “”G(1)/S transition checkpoint”" which is mediated by the tumor suppressor protein p53 has been identified. Herein, we observed
the response of activated lymphocytes to G(1)/S transition blocker to assess the G(1)/S checkpoint function and the p53 conformation state adopted in lymphocytes from AD patients and healthy non-AD controls. We found that the activated lymphocytes from AD patients were less sensitive to G(1)/S transition blocker than those from controls, indicating BI-D1870 in vivo that the G(1)/S checkpoint failed to function well in AD lymphocytes. In addition, AD cells specifically expressed an anomalous conformationally mutant-like p53 that made these cells distinct from lymphocytes of controls. We speculated that the altered conformational p53 probably be responsible
for G(1)/S checkpoint dysfunction in AD cells. Our hypothesis was supported by the results that G(1)/S checkpoint dysfunction was not restricted to neurons in AD patients, but also occurred in peripheral lymphocytes. Two potential biomarkers were indicated in blood lymphocytes from AD patients: the G(1)/S checkpoint dysfunction and the conformationally mutant-like p53 protein. (C) 2009 Elsevier Ireland Ltd. selleck kinase inhibitor All rights reserved.”
“Despite the fact that growth hormone (GH) has not been approved for antiaging purposes, its use for this indication is widespread and increasing. The Growth Hormone Research Society (GRS) convened an international workshop to critically review and debate the available evidence related to the use of GH in the older adults and the relationship between the GH/insulin-like growth factor I (IGF-I) axis and the aging process. This statement presents the conclusions reached and gives recommendations for future studies in this research field regarding the use of GH and growth hormone secretagogues (GHS) for promoting health span.